Characterization of Saccharomyces cerevisiae deficient in expression of phospholipase D.
نویسندگان
چکیده
A gene encoding phospholipase D (PLD) in Saccharomyces cerevisiae was identified. The 195 kDa product of PLD1 has 24% overall sequence identity with a plant PLD. Expression of yeast PLD activity was eliminated by one-step gene disruption. Yeast haploids lacking PLD activity were deficient in growth on non-fermentable carbon sources. Diploids lacking expression of PLD1 were unable to sporulate.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 314 ( Pt 1) شماره
صفحات -
تاریخ انتشار 1996